What is the 454 pyrosequencing platform? Explain the mechanism of three next gen-sequencing methods/platforms. These can be methods that are discussed in the Shendure paper. Compare and contrast between the three different Next gen-sequencing methods. How

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1. What is the 454 pyrosequencing platform?
2. Explain the mechanism of three next gen-sequencing methods/platforms. These can be methods that are discussed in the Shendure paper. Compare and contrast between the three different Next gen-sequencing methods.
3. How has sequencing helped in immunological studies?
4. Your thoughts on the moral and ethical aspect of companies that sequence your DNA and send you the results
5. you should use this paper https://www-nature-com.proxygw.wrlc.org/articles/nbt.2421.pdf